Creating analytical tools that support the advancement of knowledge can often come about through the integration of existing information and techniques, theoretical and practical, directed toward a ...

Ubiquitination can have profound effects on the stability and function of cellular proteins. Mass spectrometry (MS) can be used to map the specific amino acid residues that are conjugated to ubiquitin ...

Professor Kimoon Kim's research group at POSTECH has developed a highly pure and efficient technique for purifying antiviral and anti-cancer protein therapeutics using molecular affinity interaction.

Purification by affinity chromatography on immobilized calmodulin is suitable for recombinant proteins expressed in bacteria because endogenous bacterial calmodulin-binding proteins are unknown.

The changing landscape for antibody-derived therapeutics, such as bi-specific monoclonal antibodies, Fab fragments and Fc-fusion proteins brings new purification challenges in the downstream ...

Affinity chromatography theoretically does just that—a ligand that specifically interacts with the target protein is immobilized on a chromatography matrix; the target protein binds to the column, and ...

The rapid and cost-effective production of conventional monoclonal antibodies (mAbs) for clinical trials has contributed toward their wide adoption. As the number of next-generation antibody-based ...

There are a variety of uses for affinity chromatography purification techniques. They are being increasingly used to separate pharmaceutical and biological samples nowadays. Here are several ways that ...

Affinity chromatography is a separation process used to purify molecules or a group of molecules that are in a biochemical mixture. It employs two phases; a stationary phase and a mobile phase.

A novel method to improve the high-yield, high-purity, high-activity purification of complex proteins by 10- to 500-fold has been developed by scientists. The new method offers crucial advantages to ...